In vitro selection for tolerance to Fusarium in Fl microspore population of wheat. Annual Review of Phytopathology 24, 159-186.ĭeptan 2000. Tissue culture and the selection of resistant to pathogens. Seleksi in vitro untuk ketahanan terhadap penyakit layu fusarium pada tanaman abaka (Musa textilis Nee.) Tesis Program Pasca Sarjana, Institut Pertanian Bogor.ĭaud ME, 1986. In vitro manipulation and mutation breeding for the improvement of banana. 34, 287-293.Ĭhai M, YW Ho, KW Liew, A Mohd, JM Asil and AA Mohamed. Influence of Fusarium wilt toxin(s) on carnation cells. Euphytica 91,341-349.Īrai M and M Takeuchi. Culture filtrate technique and inheritance of Fusariumresistance in somaclones. In vitro techniques for selecting wheat (Triticum aesdvum L.) for Fusarzum-resistance. Acta Hort.Īhmed KZ, A Mesterhazy, A Bartok and F Sagi. In: Altman Aand Ziv M (Eds.) In vitro Culture and Breeding. Improvement of horticultural plants through in vitro c Iture and induced mutations. In vitro selection on alfalfa plants resistant to Fusariumf.sp.

Inoculation by using conidia (5 g/kg soil) provided 18 putative mutant and higher concentration of conidia (lOg/lOkg soil) produced 37 mutant that resistant to Fusarium.Īrceoni S, M Pezzotti and F Damiani F. The more dosage of irradiation and concentration of fusaric acid decreased regeneration rate of explant. However irradiation and in vitro selection caused inhibition of culture growth. The irradiation could increase somaclonal variation as well as created some new somaclones that resistant to fusaric acid. The result showed that the best medium for regeneration was MS basal medium contains 3 mg/1 BA. The selected explant about 0,5 cm were treated for 2 x 4 week selection period. On level of rad, Gamma ray radiation have been applied, continued by in vitro selection by using 0, 30 and 45 mg/1 fusaric acid. Hence, to solve the problems, mutative induction and in vitro selection to the shoot tip explants has been carried out in banana cv 'Ambon Hijau', This research was conducted at the tissue culture laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. However, facing the problems of Fusarium attack in the production centers, health seedlings in the sufficient number are difficult to be provided.

The demand on the disease free seedlings are recently increasing. Due to its high vitamin and nutrition content, banana (Musa paradisiaca L.) is deemed necessary as the mineral resources.

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